DNA fragmentation dynamics were affected by the time of incubation and reproductive season therefore, this technique might be used as an additional tool to test the potential fertility of semen samples used in feline-assisted reproduction.Ĭats undergo reproductive seasonality as an adaptive mechanism that allows for the concentration of parturitions in dates of greater food availability, thereby ensuring litter survival. The study suggests seasonal changes in some of the quality parameters of cat sperm. DNA damage was greater after 24 h of incubation at 37 ☌ in both seasons, although the percentage of spermatozoa with fragmented DNA was significantly lesser in the BS than in the NBS at 24 h (p < 0.05). No significant differences in DNA fragmentation were found between seasons after sperm collection. The total sperm motility and plasma membrane integrity values were greater during the BS, while the percentages of abnormal sperm and head defects were lesser (p < 0.05). Sperm DNA fragmentation was evaluated by the sperm chromatin dispersion test after 0, 6, and 24 h of incubation at 37 ☌. Sperm samples were obtained by cutting the cauda epididymis and assessed for sperm motility, concentration, acrosome integrity, plasma membrane integrity and sperm morphology. Epididymal spermatozoa were collected from 36 tomcats subjected to bilateral orchiectomy during breeding (BS) and non-breeding (NBS) seasons. This study was conducted to determine the effect of seasonal changes on sperm quality and on the dynamics of sperm DNA fragmentation. Feline species undergo reproductive seasonality thus, sperm characteristics, such as DNA integrity, can be affected by the photoperiod.
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